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Thus, we mutated both tyrosine 731 and 742 and expressed the double mutant (y731f/y742f) in 32d hematopoietic cells. Results are representative of three to six separate experiments. In this study, we investigated the effect of pdgf aa on cell migration, pi 3 kinase and phospholipase c (plc) activation, and the role of these two enzymes in mediating biological responses in these cells in response to all three isoforms.

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In this study we examined the association of pi3 kinase with immunoprecipitated autophosphorylated pdgf receptor in vitro Cells were preincubated with 100 nm wortmannin for 10 min The concentration of pdgf was 30 ng/ml